Journal: Experimental & Molecular Medicine

Article Title: Overexpression of Nanog in amniotic fluid–derived mesenchymal stem cells accelerates dermal papilla cell activity and promotes hair follicle regeneration

doi: 10.1038/s12276-019-0266-7

Figure Lengend Snippet: a Schematic of the process for selecting a key transcription factor to promote stemness of AF-MSCs. b Morphological changes and c growth rate of AF-MSCs infected with retroviral vectors encoding Oct4, Nanog, and Lin28, alone or in combination. Scale bar, 1 mm. d Expression of transcription factors, alone or in combination, as determined by western blotting. e Overexpression of exogenous Nanog in AF-MSCs, detected by reverse transcriptase–polymerase chain reaction (RT-PCR) and immunofluorescence. Scale bar, 1 mm. f Nanog expression in H1 embryonic stem cells, AF-MSCs overexpressing Nanog (AF-N-MSCs), and AF-MSCs. g Expression of Nanog in AF-N (infected at 10 passages) over 40 passages. h Expression of endogenous Nanog in AF-N-MSCs, detected by quantitative real-time RT-PCR. Data are represented as the mean ± SD ( n = 3). *** p < 0.001

Article Snippet: The amplified fragments were cloned into the NotI-digested pMXs vector (Cell Biolabs). pMXs vectors containing Oct4 (#17217) and Lin28 (#47902) genes were purchased from Addgene (Watertown, MA, USA).

Techniques: Infection, Retroviral, Expressing, Western Blot, Over Expression, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Immunofluorescence, Quantitative RT-PCR